pAcBac2.tR4-OMeYRS/GFP* plasmid vector桿狀病毒表達質粒載體

A single baculovirus vector encoding all of the genetic elements necessary to efficiently incorporate UAA into target proteins in mammalian cells. (A) pAcBac2 encodes a CAG promoter-driven reporter gene expression cassette, which also harbors a WPRE element at the 3′-UTR. Expression of aaRS is driven by a CMV promoter and lacks the WPRE element. (B) HEK293 cells infected with pAcBac2.tR4-OMeYRS/GFP* (MOI of 500) exhibits robust eGFP expression only in the presence of the UAA (1 mM).

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