pXC-DGV plasmid vector抗體表達質粒載體

Single gene vectors (SGVs) were con
structed using GS Xceed vectors (Lonza Biologics, Slough,
UK) (pXC IgG4 pro AK for the heavy chain constant domain
encoding region and pXC Kappa for light chain constant
domain encoding region) and the variable domain encoding regions as synthesised by GeneArt AG. The SGVs were
amplified and transiently co-transfected into Chinese Ham
ster Ovary CHOK1SV GSKO cells for initial expression at a
volume of 200 ml and then subsequently at a scaled-up vol ume of 2.5 litres.

double gene vector (DGV) was con
structed using previously established single gene vectors (see
experimental section 6 above) in Lonza’s GS Xceed vectors
(pXC IgG4 pro AK for the heavy chain constant domain
encoding region and pXC Kappa for light chain constant
domain encoding region). The DGV was amplified and stably
transfected into CHOK1 SV GS-KO cells and analysed.

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