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載體基本信息
| 出品公司: | Agilent |
|---|---|
| 載體名稱: | pFB-hrGFP |
| 質粒類型: | 逆病毒載體 |
| 高拷貝/低拷貝: | 低拷貝 |
| 克隆方法: | 限制性內切酶，多克隆位點 |
| 啟動子: | -- |
| 載體大小: | 5838 bp |
| 5' 測序引物及序列: | -- |
| 3' 測序引物及序列: | -- |
| 載體標簽: | -- |
| 載體抗性: | 氨芐青霉素 |
| 篩選標記: | 無 |
| 克隆菌株: | DH5α 等 |
| 宿主細胞（系）: | 常規細胞系（293、CV-1、CHO等） |
| 備注: | 逆病毒載體pFB-hrGFP組成型表達hrGFP綠色熒光蛋白；hrGFP細胞毒性低 |
| 產品目錄號: | -- |
| 穩定性: | 穩表達 |
| 組成型/誘導型: | 組成型 |
| 病毒/非病毒: | 逆轉錄病毒 |
載體質粒圖譜和多克隆位點信息
載體簡介
pFB-hrGFP載體限制性酶切位點pFB-hrGFP, 5838 bp version 013001Enzymes with 1-10 cleavage sites: #sites -- Bp position of recognition site -- AatII 2 978, 5764 Acc65I 3 647, 1846, 3364 AccI 3 1967, 2057, 3724 AflII 3 202, 1265, 2919 AflIII 3 164, 2157, 3953 AgeI 1 1840 AhdI 6 687, 733, 1274, 3404, 3450 4841 Alw44I 4 1054, 3769, 4267, 5513 AlwNI 6 323, 398, 2084, 3040, 3115 4364 ApaI 1 1238 ApoI 3 87, 1128, 2063 BanII 8 580, 593, 764, 1238, 2266 3297, 3310, 3481 BbeI 4 615, 1656, 2234, 3332 BbsI 3 2494, 2686, 5831 BbvCI 2 453, 3170 BciVI 5 656, 1986, 3373, 4162, 5689 BglI 1 4960 BglII 1 1678 BpmI 5 1799, 2135, 2681, 2867, 4931 Bpu10I 8 337, 412, 453, 1548, 2263 3054, 3129, 3170 BsaAI 5 1816, 2000, 2156, 2693, 3705 BsaHI 7 615, 978, 1656, 2234, 3332 5382, 5764 BsaI 10 694, 715, 782, 1414, 1802 2726, 3411, 3432, 3499, 4913 BsaWI 5 138, 1840, 4159, 4306, 5137 BseRI 6 718, 1068, 1561, 1600, 2426 3435 BsgI 2 2213, 2774 BsiEI 8 961, 1034, 1838, 2801, 3866 4290, 5213, 5362 BsiHKAI 7 580, 1054, 3297, 3769, 4267 5428, 5513 BsmBI 6 976, 1093, 1337, 1396, 1582 3602 Bsp120I 1 1238 BspHI 4 2115, 4673, 5681, 5786 BspLU11I 2 164, 3953 BsrBI 5 1299, 2798, 2823, 3884, 5685 BsrDI 2 4900, 5082 BsrGI 2 1541, 2532 BssHII 2 563, 3280 BssSI 5 2723, 2777, 4126, 5510, 5817 Bst1107I 1 3724 BstAPI 5 322, 397, 3039, 3114, 3771 BstEII 1 1346 Bsu36I 1 1276 Cfr10I 5 1840, 2099, 2348, 2504, 4926 DraI 3 4710, 4729, 5421 DraIII 1 1873 DrdI 2 3642, 4055 DsaI 4 151, 931, 2070, 2166 EaeI 7 827, 961, 1368, 1389, 1668 2801, 5234 EarI 5 1334, 1564, 2626, 3831, 5635 Ecl136II 2 580, 3297 Eco52I 2 961, 2801 Eco57I 3 2090, 4480, 5528 Eco72I 2 2000, 2156 EcoNI 2 1647, 2419 EcoO109I 5 499, 1477, 1924, 3216, 5821 EcoRI 1 2063 EcoRV 4 308, 383, 3025, 3100 EheI 4 615, 1656, 2234, 3332 FspI 2 2613, 5066 HaeII 6 615, 1656, 2234, 3332, 3827 4197 HgaI 6 889, 1166, 3659, 4055, 4633 5383 HincII 2 2057, 5385 HinfI 10 637, 1020, 1045, 1863, 2829 3354, 3853, 3928, 4324, 4841 KasI 4 615, 1656, 2234, 3332 KpnI 3 647, 1846, 3364 MscI 3 827, 1368, 1668 MslI 6 159, 2068, 2617, 5094, 5253 5612 MunI 2 11, 21 NaeI 1 2348 NarI 4 615, 1656, 2234, 3332 NcoI 2 2070, 2166 NdeI 3 1664, 1672, 3775 NgoMIV 1 2348 NheI 5 6, 16, 26, 197, 2914 NotI 1 2800 NspI 4 164, 2610, 3588, 3953 PleI 9 637, 1020, 1045, 1863, 2829 3354, 3853, 4324, 4841 PpuMI 4 499, 1477, 1924, 3216 PshAI 1 1015 Psp1406I 2 5071, 5444 PstI 5 1178, 1360, 2105, 2510, 5087 PvuI 2 1034, 5213 PvuII 7 286, 361, 2207, 2602, 2741 3003, 3078 SacI 2 580, 3297 SacII 1 151 SalI 1 2057 SapI 2 2626, 3830 SbfI 1 2104 ScaI 1 5324 SchI 9 637, 1020, 1045, 1863, 2829 3354, 3853, 4324, 4841 SexAI 1 1474 SfcI 10 182, 1178, 1360, 2105, 2510 2599, 2899, 4218, 4409, 5087 SmaI 4 643, 1241, 2018, 3360 SmlI 8 202, 1265, 2795, 2919, 4059 4321, 4598, 5466 SpeI 1 897 SrfI 1 1240 SspI 1 5648 StyI 8 496, 706, 1504, 2070, 2166 2229, 3213, 3423 TatI 5 1541, 2451, 2532, 3759, 5324 TfiI 1 3928 Tsp45I 7 1282, 1491, 2225, 3608, 3703 5103, 5314 TspRI 10 1284, 1875, 3849, 4355, 4368 4639, 4788, 4893, 5240, 5267 Tth111I 4 633, 1473, 3350, 3697 VspI 1 5017 XbaI 3 464, 2036, 3181 XcmI 1 2517 XhoI 1 2795 XhoII 10 1678, 2085, 2217, 2400, 4594 4605, 4691, 4703, 5471, 5488 XmaI 4 643, 1241, 2018, 3360 XmnI 2 2644, 5441Enzymes that do NOT cut molecule:AccIII AscI BamHI BclI BlnI Bpu1102I BsaBI BsiWI BsmI BspMI BstBI BstXI ClaI Eco47III FseI HindIII HpaI MluI NruI NsiI PacI PmeI Ppu10I RsrII SanDI SfiI SgfI SgrAI SnaBI SphI StuI SwaI Van91I 載體序列
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