Gymnascella citrina 26695

An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Product category
Fungi
Strain designation
O-1251 [CBS 375.74, NRRL 5964, NS-1094-1]
Type strain
No
Isolation source
Pig dung
Product format
Freeze-dried

Characteristics
Morphology
On freezing medium at 25°C after 5 days, mycelium white to grayish white, appears velvety but the hyphae are actually submerged in the agar, produces a white to lemon yellow fine powdery layer on the agar surface after 10 days. Hyphae hyaline, guttulate.
Comments
taxonomy

Medium 537: Freezing agar
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet.  Stir to form a suspension.
Aseptically transfer the suspension back into the test tube of sterile distilled water.
Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
Mix the suspension well.  Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
Incubate the inoculum at the propagation conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 5-6 days of incubation. However, the time necessary for significant growth will vary from strain to strain.


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