pEFC plasmid vector質(zhì)粒載體

pEFC, formed by introducing the 3' half intron, 3' splice site (3' ss), and second exon ringment of pTZ18td-2 (19) behind the tandem lac and 17 promoters, and upstream of the first exon, 5' splice site (5'ss), ad 5' half intron of pTZ18td-1 (19). Thin lines, td intron; light stippled box, exon 2 sequences from 3' splice site to end of td coding region; dark stippled box, td exon 1 sequences from start of td coding region to 5' splice site; open boxes, td flankig sequences; N, Nde I sites; X, Xba sites. (B) pEFC-ANde, formed by deleting the Nde I fragment of pEFC that spans most of the fused td exon sequences. (C) pANAct(+), formed by inserting a segment of the yeast actin gene spanning its nuclear pre-mRNA intron in the sense orientation with respect to the promoters. Solid boxes, actin exons; thin line (triangle), actin intron. (D) pA&N-Y(+), formed by placing the Xba I fragment containing all but the tandem lac and 17 promoters from the insert of pEFC-ANde into a yeast expression vector containing a GAL] promoter and the CYCI 3' end formation signals. (E) pA&N-A(+)-G(+), formed by placing all but the tandem lac and 17 promoters from the insert of pANAct(+) into pYES1.2. is used for the introduction of sequences to be included in RNA circles.  

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