FeLV-3281 cell line貓淋巴母細胞株攜帶貓白血病病毒A型

Organism
Felis catus, cat
Morphology
lymphoblast

Growth properties
Suspension
Genes expressed
feline leukemia virus (FeLV), subgroup A

Unpacking and storage instructions
1.Check all containers for leakage or breakage.
2.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium
RPMI 1640 medium with 4 mM L-glutamine and 0.025 mg/ml gentamicin sulfate, 90%; fetal bovine serum, 10%
Handling procedure
HANDLING PROCEDURE FOR FROZEN CELLS

- Initiate culture as soon as possible upon receipt.

- Thaw by rapid agitation in 37°C water bath. Thawing should be rapid (within
40-60 seconds). As soon as the ice is melted, remove the ampule from the water
bath. All of the operations from this point on should be carried out under
strict aseptic conditions.

- Transfer the cell suspension and dilute it with the recommended culture
medium in a culture flask (see specific batch information above for dilution
ratio); incubate at 37°C with 5% CO2 in air atmosphere. Since it is important
to avoid excessive alkalinity of the medium during recovery of the cells, it is
suggested that the culture medium be placed into the culture flask, tube, etc.
and the pH be adjusted, as necessary, prior to the addition of the vial
contents. Note that the bicarbonate content of the culture medium will
determine whether an atmosphere containing CO2 will be required.

- It is not necessary to remove the freezing additive. However, if desired,
the culture medium may be changed to remove the protective freezing additive
(dimethylsulfoxide) 24 hours after thawing. If it is desired that the freezing
additive be removed immediately, or that a more concentrated cell suspension be
obtained, centrifuge the above diluted suspension at approximately 125 x g for
10 minutes, discard the fluid and resuspend the cells with growth medium at the
dilution ratio given in the specific batch information above.

FLUID RENEWAL
Every 2-3 days.

SUBCULTURE PROCEDURE
Cultures can be maintained by the addition of fresh medium or replacement of
medium. Alternatively, cultures can be established by centrifugation with
subsequent resuspension at 10(5) viable cells/ml. Maintain cell density between
10(5) and 10(6) per ml.
Subculturing procedure
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Reagents for cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO

Mycoplasma contamination
Not detected

Supplier來源:BioVector NTCC Inc.
TEL電話:400-800-2947
Website網址: http://www.biovector.net