Name：A549-Dual? KO-RIG-I Cells RIG-I knockout NF-kB-SEAP & IRF-Lucia Reporter Cell Line
Description描述：A549 lung carcinoma A549-Dual? cells are adherent epithelial cells that have been derived from the human A549 lung carcinoma cell line by stable integration of two inducible reporter constructs. The A549 cell line is a well-characterized cellular model for asthma, allergies and respiratory infections. A549-Dual? cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IFN-β minimal promoter fused to five NF-κB binding sites. A549-Dual? cells also express the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. As a result, A549-Dual? cells allow to simultaneously study the NF-κB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue?, a SEAP detection reagent, and QUANTI-Luc?, a Lucia luciferase detection reagent.
ID編號:
Size/Quantity數(shù)量:1 vial
Biosafety Level生物安全級別: 1
Shipping Info運(yùn)輸方式: Dry Ice
Storage儲(chǔ)存方式: Liquid Nitrogen
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Complete Growth Medium完全培養(yǎng)基:
Subculturing傳代方法:
Cryopreservation凍存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培養(yǎng)條件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鑒定數(shù)據(jù):
References參考文獻(xiàn):

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