Name質(zhì)粒名稱:pDRIVE-HSP70 HSP70 promoter (Ubiquitous, heat inducible) in pDRIVE expression plasmid
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Description描述:Ubiquitous Promoters Ubiquitous Promoters are strongly active in a wide range of cells, tissues and cell cycles. Ubiquitous Promoters are available as native or composite promoter: Native promoters, also called minimal promoters, consist of a single fragment from the 5’ region of a given gene. Each of them comprises a core promoter and its natural 5’UTR. In some cases, the 5’UTR contains an intron. Composite promoters combine promoter elements of different origins (e.g. SV40 enhancer/AFP promoter) or were generated by assembling a distal enhancer with a minimal promoter of the same origin (e.g. CEA enhancer/promoter). In each pDRIVE, the promoter drives the expression of a reporter gene to facilitate the evaluation of its activity. Three different reporter genes are available in two different backbones: LacZ reporter gene in pDRIVE, secreted embryonic alkaline phosphatase (SEAP) or Lucia secreted luciferase reporter gene in pDRIVE5.
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