Name質粒名稱:Original (D614) Original Spike (D614) Pseudotyping vector
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Description描述:Spike Pseudotyping Vectors SARS-CoV-2 spike (S)-pseudotyped lentiviral particle production Production of SARS-CoV-2 spike (S)-pseudotyped lentiviral particles using InvivoGen's pLV-Spike plasmids InvivoGen also offersINVIVOGEN ALSO OFFERS: ? SARS-CoV-2-Cellular Receptor Genes ? HEK293 ACE2-expressing Cell Line ? A549 ACE2-TMPRSS2-expressing Cell Line Original (D614) and variants of concern InvivoGen offers a collection of plasmids specifically designed for pseudotyping lentiviral particles with the full-length SARS-CoV-2 Spike (S) protein. For optimal expression, the spike gene has been codon-optimized and the C?terminal ER-retention signal has been removed. Various SARS-CoV-2 variants are available: Original | Wuhan (Clade 19) G614 variant | (Clade 20) Alpha | B.1.1.7 variant | United Kingdom (Clade 20I) Beta | B.1.351 variant | South Africa (Clade 20H) Epsilon | B.1.429 variant | California (Clade 21C) Gamma | P.1 variant | Brazil (Clade 20J) Iota | B.1.526 variant | New York (Clade 21F) Kappa | B.1.617.1 variant | India (Clade 21B) Delta | B.1.617.2 variant | India (Clade 21A) Lambda | C.37 variant | Peru (Clade GR/452Q.V1) Mu | B.1.621 variant | Colombia (Clade 21H) Omicron | B.1.1.529/BA.1 variant | South Africa (Clade 21K) Omicron | BA.2 variant | South Africa (Clade 21L) Click here for more information on SARS-CoV-2 variants Application Pseudotyped particle production involves the co-transfection of 293T cells with a reporter protein vector (e.g. GFP), one or several plasmids encoding the necessary lentiviral proteins, and InvivoGen's pseudotyping pLV-Spike plasmids. The transfected cells produce SARS?CoV?2 Spike (S)?pseudotyped lentiviral particles, which can then be purified and used to infect permissive cells, such as ACE2?expressing HEK293-derived cells and ACE2?TMPRSS2?expressing A549-derived cells.
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