Name質粒名稱:pUNO1Fc-SARS2-N
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Description描述:SARS-CoV-2 Nucleocapsid, codon-optimized & tagged in C-term Schematic of tagged SARS2 nucleocapsid protein production vectors Schematic of tagged SARS2 nucleocapsid production vectors InvivoGen also offersINVIVOGEN ALSO OFFERS: ? SARS-CoV-2-Cellular Receptor Genes ? SARS-CoV-2-Structural Genes GENE DESCRIPTION The SARS-CoV-2 (2019-nCov) Nucleocapsid (N) is a phosphoprotein that associates with the viral RNA genome and forms the ribonucleoprotein core. This structural protein plays a number of important roles in the viral life cycle including replication, transcription, and genome packaging. These functions are mediated by two principal domains located at the N- and C-terminal termed NTD and CTD, respectively [1-6]. pUNO1His-SARS2-N and pUNO1Fc-SARS2-N express a codon-optimized nucleocapsid sequence fused to a C-terminal His- or Fc-tag, respectively. Both plasmids contain the nucleocapsid coding sequence from the Wuhan-Hu-1 isolate. (See Specifications for more information) PLASMID DESCRIPTION pUNO1His-SARS2-N and pUNO1Fc-SARS2-N are designed for the production and secretion of a tagged nucleocapsid protein. These vectors feature a potent mammalian expression cassette comprised of the strong SV40 enhancer, the ubiquitous human EF1α-HTLV composite promoter, and the SV40 polyadenylation (pAn) signal. The codon-optimized nucleocapsid coding sequence is preceded by an exogenous signal sequence to ensure effective protein secretion. Both plasmids are selectable with blasticidin in E. coli and mammalian cells.
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