MCFS cell line小鼠成纖維細胞

The cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes, are a source of paracrine growth factors, and are an important cellular component of myocardial responses to injury. CF are responsible for extracellular matrix synthesis in the heart during development and pathological conditions. Their proliferation and matrix synthesis is essential for scar formation at sites of myocardial infarction [1], cardiac fibrosis, and is often complicated by cardiac hypertrophy [2]. CF also respond to changes in microenvironments in pathological conditions by modulating integrin expression [3]. CF culture has been widely used as a model to study the cardiac matrix remodeling by physiological (exercise) and pathological (hypertension) stressors.
MCF from BioVector Research Laboratories are isolated from postnatal day 2 C57BL/6 mouse heart. MCF are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MCF are characterized by immunofluorescence with antibody specific to fibronectin. MCF are negative for mycoplasma, bacteria, yeast, and fungi. MCF are guaranteed to further expand for 5 population doublings under the conditions provided by BioVector Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing MCF in vitro.

Country of Manufacture United States
Product Code MCFs
Size/Quantity 5 x 10^5 cells/vial
Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping Info Dry ice.
References [1] Sabri A, Short J, Guo J, Steinberg SF. (2002)

Supplier來源:BioVector NTCC Inc.
TEL電話:400-800-2947
Website網址: http://www.biovector.net