M3E3/C3 cell line細(xì)胞株

Cell line:
M3E3/C3
DSMZ no.:
ACC 340
Species validation:
Syrian hamster (golden hamster) (Mesocricetus auratus)
Cell type:
fetal lung
Origin:
established from the lung of a Syrian golden hamster fetus on day 15 after gestation in 1981; primary culture cells were treated with hydroxy-proline; cells were described as premature epithelial cells with the ability to differentiate into mucus-producing pulmonary cells
Reference(s):
14511
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
epithelial-like cells growing in monolayers
Medium:
90% RPMI 1640 + 10% h.i. FBS + 1 mM sodium pyruvate
Subculture:
split confluent culture 1:3 to 1:6 every 3-4 days using trypsin/EDTA; seed out at 1-2 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of ca. 8 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mesocricetus auratus species.
Cytogenetics:
near-diploid Syrian hamster karyotype with 10% polyploidy - 43-47<2n>
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -

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