pK19ProteinA-MNase plasmid融合表達Protein A和金葡菌MNase原核表達質粒

ChIC; genomic mapping of chromatin proteins
This plasmid expresses a pA-MN fusion protein in E. coli. The MN coding sequence was cloned into pK19SEVII (Giraud-Panis et al., 1995). The pA segment contains 2 IgG binding domains of staphylococcal protein A and was fused to MN with a linker sequence of DDDKEF.

Vector typeBacterial Expression
GROWTH IN BACTERIA
Bacterial Resistance(s)Kanamycin
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberUnknown
GENE/INSERT
Gene/Insert nameprotein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
SpeciesStaphylococcus aureus
Insert Size (bp)927
GenBank IDGenbank P00644 GenBank: CP018205.1


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