pTXB-Tn5 Transposase plasmid Tn5轉座酶原核表達質粒

IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

Vector typeBacterial Expression
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin
Growth Temperature37°C
Growth Strain(s)XL1 Blue
Growth instructionsGrowth at 37°C before IPTG induction. After IPTG addition, grow at 30°C. The depositing laboratory recommends E. coli strains NEB C3013 or ER2566 for protein expression.
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nametnpA from pBam1
Alt nameTn5 transposase
SpeciesE. coli
Insert Size (bp)1428
MutationE54K, L372P
GenBank IDHQ908071.1
PromoterT7 promoter
Tag / Fusion Protein
Mxe intein - Chitin-binding domain (C terminal on insert)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteXba (not destroyed)
3′ cloning siteLgu1/SapI) (destroyed during cloning)
5′ sequencing primerT7 promoter primer
3′ sequencing primerGATTGCCATGCCGGTCAAGG

Supplier來源:BioVector NTCC Inc.
TEL電話:400-800-2947
Website網址: http://www.biovector.net