表達hla-g的721.221 cell line細胞株LCLg

To detect HLA-G dimers, surface proteins of 721.221 cells transfected with HLA-G1 cDNA were biotinylated and immunoprecipitated with a panel of HLA-I-reactive antibodies. HLA-G-specific MEM-G/11, 87G and G233 recognise the 尾2m-associated class I structure. W6/32 is also conformationally dependant but binds all HLA-I molecules. The immunoprecipitated complexes were first analysed by denaturing reducing PAGE, followed by Western blotting for biotin-labelled surface proteins. As expected, both HLA-G-specific and pan-class I antibodies detected an HLA-G band at 39 kDa, as well as co-immunoprecipitating the 13.7-kDa 尾2m molecule

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