pIncW-T7 RNAP plasmid原核表達質粒IPTG誘導表達T7 RNAP質粒

expresses an attenuated T7 RNAP under an isopropyl 尾-d-1-thiogalactopyranoside (IPTG) inducible control

Vector typeBacterial Expression, Synthetic Biology

Bacterial Resistance(s)Spectinomycin
Growth Temperature37°C
Growth Strain(s)DH10B
Growth instructionsPlasmid is maintained at only 2-3 copies/cells, so it is difficult to sequence from minipreps (better to use PCR).
Copy numberLow Copy

Gene/Insert name T7 RNAP*
Alt nameattenuated T7 RNAP
SpeciesBacteriophage T7
MutationR632S
Promoterp Tac-symmetric
Tag / Fusion Protein
umuD degradation tag (N terminal on backbone)

Supplier來源:BioVector NTCC Inc.
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