K562-Cd38 cell line靶點穩定重組細胞株

CD38 is a glycoprotein with ectoenzymatic functions, which is expressed on plasma cells and other lymphoid and myeloid cell populations. Since its expression is very high and uniform on myeloma cells, CD38 is a good target for novel therapeutic strategies. Among them, immunotherapy represents a promising approach.
CD38 is a 45 KDa surface glycoprotein, firstly identified as an activation marker: successively the molecule was reported as an adhesion molecule, able to interact with endothelial CD31. These finding highlighted the possibility that CD38 may act as a receptor, notwithstanding a structural ineptitude to do so. It was shown indeed that CD38 act as an accessory component of the synapse complex . CD38 was then identified as an ectoenzyme involved in the metabolism of extracellular nicotinamide adenine dinucleotide (NAD+) and cytoplasmic nicotinamide adenine dinucleotide phosphate (NADP). The results is the production of Ca2+-mobilizing compounds, such as cyclic adenosine diphosphate [ADP] ribose, ADP ribose (ADPR) and nicotinic acid adenine dinucleotide phosphate. CD38 enzymatic activities were shown as able to rule the NAD+ levels and improve the function of proteasome inhibitors. Further, ADPR produced by CD38 can be further metabolized by the concerted action of CD203a/PC-1 and CD73, to produce the immunosuppressive molecule adenosine (ADO). This feature points out the role of CD38 in the escape of tumor cells from the control of the immune system.

The K562-CD38 recombinant cell line has been transfected with full-length human CD38 cDNA under control of a CMV promoter for high constitutive expression.

Application(s): Functional(Report Gene) Assay
Organism: Homo sapiens, human
Tissue: bone marrow
Disease: chronic myelogenous leukemia (CML)
Age: 53 years
Gender: female
Morphology: lymphoblast
Growth Properties: suspension

Cytogenetic Analysis:
The stemline chromosome number is triploid with the 2S component occurring at
4.2%. Fifteen markers (M1 and M(15)) occurred in nearly all S metaphases. Spontaneous nonspecific
dicentrics occurred, but rarely. Unstable markers were also rarely seen. The X was disomic, and N9 was nullisomic.

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