HCT116-5-FU cell line耐藥細(xì)胞株 BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心

價(jià)　　格：￥498685
貨　　號(hào)：HCT116-5-FU
產(chǎn)　　地：北京

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HCT116-5-FU cell line耐藥細(xì)胞株

The 5-FU resistant HCT116 cell line (HCT116/5-FU) was established by repeated exposure to gradually increasing concentrations of 5-FU; Cell viability was measured by cell counting kit-8 (CCK-8); apoptosis rate of HCT116 cells was detected using Annexin V-fluorescein isothiocyanate (FITC) assay kit; cell proliferation and invasion were detected using colony formation assays, wound healing assay and transwell invasion assays; activity of transplanted tumor in vivo in specific pathogen free (SPF) BALB/c nude mice (6 weeks old, male) was monitored by bioluminescence imaging, immunohistochemistry and western blot analysis.

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