XL1-Blue MRF' Electro-competent cells E.coli大腸桿菌電轉感受態細胞

Genotype and Background
Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)]. (Genes listed signify mutant alleles. Genes on the F′ episome, however, are wild-type unless indicated otherwise.)

The XL1-Blue MRF′ (Minus Restriction) strain is a restriction minus (McrA-, McrCB-, McrF-, Mrr-, HsdR-) derivative of Stratagene’s XL1-Blue strain. XL1-Blue MRF′ cells are deficient in all known restriction systems [Δ(mcrA)183, Δ(mcrCB-hsdSMR-mrr)173], and are endonuclease (endA), and recombination (recA) deficient. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system, and the recA mutation helps ensure insert stability. The endA mutation greatly improves the quality of miniprep DNA. The lacIqZΔM15 gene on the F′ episome allows blue-white screening for recombinant plasmids.

Antibiotic Resistance
XL1-Blue MRF' cells are resistant to tetracycline.

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