EOL-1 cell line人嗜酸粒細胞白血病細胞株

Cell Line Name: EoL-1 cell
BioVector NTCC Inc.

Keywords: Human eosinophilic leukaemia
Cell Line Description: Differentiated by treatment with N-butyrate.
Species: Human
Tissue of Origin: blood
CellType: Lymphoblast
Growth Mode: Suspension
DNA Profile: STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 12,13
D16S539: 8,10
D5S818: 11
D7S820: 10,13
THO1: 9.3
TPOX: 8
vWA: 16,17

Karyotype: Not specified
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Eol-1 cells grow very slowly and may take up to 5 days to reach a suitable level to subculture. It is best that these cells are left to grow and not agitated more than necessary.The cells actually grow as large clusters. They are beginning to reach confluence when the cells in the centre of each cluster start to look unhealthy. The change in media colour should also be an indication. Eol-1 cells prefer slightly acidic conditions and so may resuscitate better from frozen if started up in 15% foetal bovine serum.
Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Supplier來源:BioVector NTCC Inc.
TEL電話:400-800-2947
Website網址: http://www.biovector.net