amj2-c11 cell line小鼠肺泡巨噬細(xì)胞株

AMJ2-C11
Cat#BioVector925856

Product category
Animal cells
Organism
Mus musculus, mouse
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
Cell type
macrophage, alveolar
Morphology
macrophage
Tissue
Lung
Applications
3D cell culture
Immunology

Characteristics
Growth properties
Mixed: suspension with some loosely adherent cells
Derivation
AMJ2-C8 (NTCC-932455) and AMJ2-C11 (NTCC-932456) are cloned, continuous, alveolar macrophage (AM) cell lines generated from C57BL6J mice by in vitro infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes.
Age
10 weeks
Gender
Female
Strain
C57BL/6J
Antigen expression
MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; Ly-5 +; Thy-1 -; Lyt-1 -
Genes expressed
interleukin-6 (interleukin 6, IL-6)
Comments
AMJ2-C8 (NTCC-932455) and AMJ2-C11 (NTCC-932456) are cloned, continuous, alveolar macrophage (AM) cell lines generated from C57BL6J mice by in vitro infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes.

Flow cytometry detected the product of the raf gene in the cytoplasm of these cell lines.

Studies on the tumoricidal properties of these cell lines demonstrated differences in their response to a panel of known macrophage activators.

AMJ2-C8 was activated following exposure to recombinant murine interferon gamma (rMuIFN-gamma) but not lipopolysaccharide (LPS) or muramyl dipeptide (MDP).

AMJ2-C11 most closely resembled the response pattern of the parental AM, since it could be activated by either the combination of rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP.

The cells retain many characteristics of alveolar macrophages. They are phagocytic, non-specific esterase positive and they express macrophage Mac-1 antigens and Fc receptors.

Constitutive expression of MHC-class-II antigens was low on AMJ2-C11 but was increased following exposure to rMuIFN-gamma.

The cell line did not secrete substantial amounts of IL-1 or TNF but did secrete large amounts of IL-6.

The cells produce nitric oxide (NO) when stimulated with a mixture of rMuIFN-gamma and LPS.






Handling information
Unpacking and storage instructions
Check all containers for leakage or breakage.
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).
Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
Transfer the vial contents to a centrifuge tube containing  9.0 mL complete culture medium and spin at approximately 125 x g for 5 to 10 minutes.
Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a 25 cm2 or a 75 cm2 culture flask.  It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.
Subculturing procedure
Firmly tap the flask against palm of hand to dislodge any attached cells and transfer along with the floating cells into new flasks.

Medium Renewal: Twice per week
Reagents for cryopreservation
Complete growth medium supplemented with 5% (v/v) fetal bovine serum (ATCC 30-2020) and 5% (v/v) DMSO

Supplier來源:BioVector NTCC Inc.
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