fck-Jaws-GFP-ER2 plasmid慢病毒表達質粒

BACKBONE
Vector backbone FCK(1.3)GW
(Search Vector Database)
Backbone manufacturerPavel Osten
Backbone size w/o insert (bp)9224
Total vector size (bp)10828
Vector typeMammalian Expression, Lentiviral
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)Stbl3
Growth instructionsPlease note that this backbone is highly recombination prone and should be amplified in a rec- strain like Sure2 or Stbl3, using carbenicillin rather than ampicillin.
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameJaws-GFP-ER2
Alt nameJaws-ER2
SpeciesHaloarcula salinarum (strain Shark)
Insert Size (bp)1584
MutationK200R W214F
GenBank IDKM000927.1
PromoterCaMKII
Tags / Fusion Proteins
GFP (C terminal on insert)
ER2 (C terminal on insert)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteBamHI (not destroyed)
3′ cloning siteEcoRI (not destroyed)
5′ sequencing primerCAC ACA GTC CTG CAG TAT TGT GTA TAT
3′ sequencing primerCAA GGA GGA GAA AAT GAA AGC C

Supplier來源:BioVector NTCC Inc.
TEL電話:400-800-2947
Website網址: http://www.biovector.net