pMSCV-Cas9-2A-GFP-sgRNA plasmid vector逆病毒基因編輯質(zhì)粒載體

Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector
BACKBONE
Vector backbonepMCSV
(Search Vector Database)
Backbone size w/o insert (bp)3000
Total vector size (bp)10500
Vector typeRetroviral
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)NEB Stable
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameCas9
Alt nameS. pyogenes CRISPR-Cas9
SpeciesSynthetic; Streptococcus Pyogenes
Insert Size (bp)4200
PromoterMSCV
Tag / Fusion Protein
2A-GFP (C terminal on insert)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteXhoI (not destroyed)
3′ cloning siteEcoRI (not destroyed)
5′ sequencing primerGag

Supplier來源:BioVector NTCC Inc.
TEL電話:400-800-2947
Website網(wǎng)址: http://www.biovector.net