pSB-EF1a-GFP-Neo哺乳動物睡美人轉座子熒光表達質粒載體

Empty Backbone.Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters.
The EF1a-GFP cassette was isolated from the plasmid pRRLsin.PPTs.EF1a.GFPpre (Bonamino et al., 2004) (provided by Dr. Didier Trono, EPFL, Switzerland) after digestion with ClaI/BstBI and inserted in pT3-Neo previously digested with ClaI.

EGFP has additional 10 amino acids on the c-terminus of EGFP (SGLRSRLASS). Depositor states that these amino acids do not affect plasmid function.

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