Syn61Δ3(ev5) ΔrecA (ev1) strain大腸桿菌突變菌株

Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

GROWTH IN BACTERIA
Bacterial Resistance(s)Streptomycin, 50 μg/mL
Growth Temperature37°C
Growth Strain(s)Syn61Δ3(ev5) ΔrecA (ev1)
Growth instructionsGrow in LB, SOB, or 2×YT at 37°C
Copy numberUnknown
GENE/INSERT
Gene/Insert nameΔrecA
SpeciesSynthetic
MutationΔrecA
CLONING INFORMATION
Cloning methodUnknown
5′ sequencing primerGAGTTTACGTCGCAGTTCTTG
3′ sequencing primerACTGAAAGCGGCTCGTGCT

Supplier來源:BioVector NTCC Inc.
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