Faecalibaculum rodentium strain菌株

Name:
Faecalibaculum rodentium Chang et al. 2016 emend. Cox et al. 2017
NTCC? No.:
103405, Type strain
Strain designation:
ALO17

Isolated from:
faeces, female C57BL/6 J mouse
Country:
Republic of Korea
Date of sampling:
11.03.2014

History:
<NTCC <-B.C. Kim, South Korea; ALO17
Genbank accession numbers:
16S rRNA gene: KP881689
complete genome: CP011391

Cultivation conditions:
NTCC? Medium 104 , strictly anaerobic, 37°C

Please follow special instructions: 'Cultivation of Anaerobes'

NTCC? Medium 104. PYG MEDIUM (modified)
Trypticase peptone 5.00 g
Peptone 5.00 g
Yeast extract 10.00 g
Beef extract 5.00 g
Glucose 5.00 g
K2HPO4 2.00 g
Tween 80 1.00 ml
Salt solution (see below) 40.00 ml
Resazurin 1.00 mg
Vitamin K1 solution (see below) 0.20 ml
Haemin solution (see below) 10.00 ml
Cysteine-HCl x H2O 0.50 g
Distilled water 950.00 ml
The vitamin K1, haemin solution and the cysteine are added after the medium has been
boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and
autoclave.
Salt solution:
CaCl2 x 2 H2O 0.25 g
MgSO4 x 7 H2O 0.50 g
K2HPO4 1.00 g
KH2PO4 1.00 g
NaHCO3 10.00 g
NaCl 2.00 g
Distilled water 1000.00 ml
Haemin solution:
Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water. Store
refrigerated.
Vitamin K1 solution:
Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated
in a brown bottle.

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