A2780cis cell line人卵巢癌順鉑耐藥細胞株 BioVector NTCC質粒載體菌種細胞基因保藏中心
- 價 格:¥989865
- 貨 號:A2780cis
- 產 地:北京
- BioVector NTCC典型培養物保藏中心
- 聯系人:Dr.Xu, Biovector NTCC Inc.
電話:400-800-2947 工作QQ:1843439339 (微信同號)
郵件:Biovector@163.com
手機:18901268599
地址:北京
- 已注冊
A2780cis cell line人卵巢癌順鉑耐藥細胞株
Cell Line Name: NTCC? A2780cis
Keywords: Human ovarian carcinoma
Cell Line Description: This cisplatin-resistant cell line has been developed by chronic exposure of the parent cisplatin-sensitive A2780 cell line (NTCC? 93112519) to increasing concentrations of cisplatin. A2780cis is cross-resistant to melphalan, adriamycin and irradiation. An increased ability to repair DNA damage as well as cytogenetic abnormalities has been observed. In order to retain resistance cisplatinum has to be added to the media for every passage. In addition to this matched pair of drug-sensitive/resistant cell lines an adriamycin-resistant cell line, A2780adr (NTCC?. 93112520), has been isolated from the same parental line A2780.
Species: Human
Tissue of Origin: ovary
CellType: Epithelial
Growth Mode: Adherent
Karyotype: Modal no. 46
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x10,000cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells will attach slowly after resuscitation and take up to 7 days to reach confluency. Recommendation: resuscitate cells in media without cisplatin. Add after subculture of attached cells.
Culture Medium: RPMI 1640 + 2mM Glutamine + 1μM cisplatinum + 10% Foetal Bovine Serum (FBS) .
Image
Supplier來源:BioVector NTCC Inc.
TEL電話:400-800-2947
Website網址: http://www.biovector.net
- 公告/新聞