Bhas 42 cell line細(xì)胞株

Cell Name   Bhas 42
Profile   BALB 3T3 A31-1-1 cell line transfected with H-ras gene. The cell line keeps characteristics of the contact inhibition and the transfomation is induced by phorbol ester alone. No initialization required. (Strain problem was found. Refer Important Notice at cell detail page.) Other Name  
Animal   mouse Strain   Swiss
Genus   Mus Species   musclus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history   normal Metastasis  
Tissue Metastasized    Genetics   H-ras gene transfected BALB/c 3T3 A31-1-1 cell line. The origin of BALB/c 3T3 A31-1 was found not to be derived from BALB 3T3 A31.
Life Span   infinite Crisis PDL  
Morphology   fibroblast-like Character   Growth on the dish is inhibited by cell to cell contact. Phorbol ester alone is enough to induce cellular transformation.
Classify   transfected Established by   Sasaki,K.
Registered by   Sasaki,K. Regulation for Distribution   free
Comment   Appearing of naturally occured transformation depends on serums and passage methods. Year   2000
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA. Split ratio is practically 1/100. Harvest cells before confluent at subculture.
Cell Number on Passage   2 x 10^4 cells/100mm dish. Race  
CO2 Conc.   5 % Tissue Sampling   whole body of embryo
Tissue Type  
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Supplier來源:BioVector NTCC Inc.
TEL電話:400-800-2947
Website網(wǎng)址: http://www.biovector.net