NEB10-beta E.coli strain / competent cells大腸桿菌菌株/感受態細胞

Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.

重點
DH10B? derivative
Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Resistance to phage T1 (fhuA)
Suitable for blue/white screening without IPTG by a-complementation of the b-galactosidase gene
Reduced recombination of cloned DNA (recA1)
Clone large plasmids and BACs
Free of animal products
K12 Strain
Transformation Efficiency
For C3019I and C3019H:  1–3 x 109 cfu/μg pUC19 DNA
For C3019P: 1–3 x 108 cfu/μg pUC19 DNA
基因型
Δ(ara-leu) 7697 araD139  fhuA ΔlacX74 galK16 galE15 e14-  ?80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC)

Supplier來源:BioVector NTCC Inc.
TEL電話:400-800-2947
Website網址: http://www.biovector.net