NTCC? KILR CD16 Effector Cells are purified human cytotoxic T lymphocytes stably expressing CD16 (Fc?RIIIa; V158 variant), produced in a standardized batch. The cells were developed for antibody-dependent cell-mediated cytotoxicity (ADCC) applications where low lot-to-lot variability is required, such as lot release of therapeutic antibodies.

Receptor Species

Human

Cell Type

Oligoclonal human cytotoxic T lymphocytes

Assay Measures

Cellular Cytotoxicity

Culture Mode

Suspension

Storage

Store in vapor phase of liquid N2.

Highlights of KILR CD16 Effector Cells

• Eliminate Donor Variability – Primary effector cells (human cytotoxic T lymphocytes) from a single donor engineered to stably express CD16 (V158)

• Fit for Long-Term QC Testing – Uniformly manufactured to ensure lot-to-lot consistency and produce high S/B ratios

• Measure Target Cell Death – High killing capacity for implementing ADCC or TCR for lot release or characterization

Single Donor-Derived Effector Cells

Single donor-derived cytotoxic T lymphocytes (CTLs) transfected with CD16 (FcγRIIIa-V158). KILR CD16 Effector Cells are engineered to express CD16 receptor to form an immunological synapse between the target antibody and antigen that ultimately triggers the release of perforins, granzymes, and cytokines that promote cell death when delivered to target cell cytosol. A. KILR CD16 Effector Cells showing a polyclonal population of predominantly (>98%) CD8+ cells and (B.) positive for CD3 with robust and stable expression of CD16. C. KILR CD16 Effector Cells have functional CD3 as demonstrated by the ability of cells to stimulate T-cell redirection in the presence of blinatumomab (see the section on T-cell redirection).

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Excellent Intermediate Precision of ADCC and High Lot-to-Lot Reproducibility

Evaluation of the intra- and inter-lot performance of KILR CD16 Effector Cells in a Rituximab ADCC model. High lot-to-lot reproducibility ensures long-term support for QC testing.

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