NTCC? Primary Human Neuron Cells原代人神經元細胞

Name of Products NTCC? Primary Human Neuron Cells
Catalogue Number NTCC?-HNC001
Product Format Frozen Vial
Cell Number > 3x10[5] cells/vial

GENERAL INFORMATION: HNCs are initiated by digestion of minced brain cortical tissue with collagenase. HNCs
are separated from the mixture of cell populations and offered at passage 1 in a frozen vial. HNCs Growth Medium
(contains 10% serum and growth supplements).

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CHARACTERIZATION OF THE CELLS
1. Neurofilament protein Positive
2. Neuron specific enolase (NSE) Positive
3. Glial fibrillary acidic protein (GFAP) Negative
4. Myelin basis protein (MBP) Negative
5. HNCs are negative for HIV-1, HBV, HCV, and mycoplasma.

SHIPPING Frozen Vials on Dry Ice.
HANDLING OF ARRIVING CELLS
When you receive the dry ice package with cells in frozen vials, transfer the
frozen vials of cells into a -80C freezer for short period storage or a liquid nitrogen tank for long- term storage.

PROTOCOLS FOR THAWING:
1. Pre-coating of T25 flasks- Add 2ml of AlphaBiocoat into a T25 flask to cover the whole surface of the flask,
Place the flasks in a 37C incubator for 30 minutes. After 30 minutes, remove your coated flask from the
incubator, dispose the excessive coating solution by aspiration under a biosafety cabinet. Rinse the T25
flask twice with 1x PBS and the flask is ready to be used.
2. Thaw the frozen cell vial in a 37C water bath first, and then transfer the cells into the pre-coated T25 flask
with 10ml of Neuro Growth media, cells usually become confluent with 10-12 days.

SUBCULTURING THE CELLS
Note: If you have any questions or need clarification regarding the protocol for culturing these cells, please reach out to Dr. Jensen Auguste at (978) 608-1766 with your questions before beginning.
3. Pre-coat 2 or 3 T25 flasks- Add 2ml of AlphaBiocoat into each T25 flask to cover the whole surface of the
flask. Place the flasks in a 37C incubator for 30 minutes. After 30 minutes, remove your coated flask from
the incubator, dispose the excessive coating solution by aspiration under a biosafety cabinet. Rinse the T25
flask twice with 1x PBS and the flask is ready to be used.
4. To passage the cells, rinse the cells in the T25 flask with 5ml 1x PBS (RT) twice; discard the 1x PBS, then
add 2ml Trypsin/EDTA (RT) to the T25 flask.
5. Place the T25 flask with the cells in a 37C incubator for 1 min (most cells usually will detach from the surface
within 1-2 mins; or monitor the cells under a microscope until most of cells become rounded up and are
detach from the flask.
6. Add 5ml of Trypsin Neutralization Buffer or Neuro Growth media spin down the cells at 800g centrifugation
for 5 mins. Once the five minute is completed, under a biosafety cabinet discard old medium.
7. Re-suspend the cell pellet with 10 or 15ml Neuro Growth media and transfer 5 ml each into 2 or 3 *precoated T25 flasks (for 1/2 to 1/3 subculture ratio).
8. Change medium every 2 or 3days and the cells usually become confluent within 10-12 days (when split at a
1/3 ratio). It is recommended that these cells have a minimum average population doubling capacity > 8
when cultured following our detailed protocol).

Supplier來源:BioVector NTCC Inc.
TEL電話:400-800-2947
Website網址: http://www.biovector.net