• Vector backbone
  pEGFP-C2
• Backbone manufacturer
  Clontech
• Vector type
  Mammalian Expression
• Selectable markers
  Neomycin (select with G418)

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Kanamycin, 50 μg/mL
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  Unknown

GENE/INSERT

• Gene/Insert name
  TEM4
• Alt name
  ARHGEF17
• Alt name
  P164RHOGEF
• Species
  H. sapiens (human)
• Mutation
  L722F
• GenBank ID
  NM_014786.3NP_055601.2
• Entrez Gene
  ARHGEF17(a.k.a. P164RHOGEF, TEM4, p164-RhoGEF)

• PromoterCMV

• Tag / Fusion Protein

  • EGFP (N terminal on backbone)

  
  

CLONING INFORMATION

• Cloning methodRestriction Enzyme

• 5′ cloning siteEcoRI (not destroyed)

• 3′ cloning siteXbaI (not destroyed)

• 5′ sequencing primerEGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3')

• 3′ sequencing primerSV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')