Physical map of pMW1650. The transposase, Himar1, is under the control of the borrelial promoter, flgBp (16). The region including rpsLp-arr-2_Rp (Rif^r), ompAp-GFP_UV, and ColE1 represents the transposable element and is bounded by inverted repeats (IR), denoted by triangles. The small arrows indicate oligonucleotides (DW317 and DW1100) used for subsequent sequencing after rescue in E. coli. ColE1, an E. coli origin of replication; Kan^r, the kanamycin resistance gene.