pBacPAK8 plasmid, BacPAK Baculovirus Expression System

pBacPAK8 is a transfer vector designed for high-level expression of a cloned gene driven by the strong AcMNPV

polyhedrin promoter. Flanking AcMNPV sequences allow recombination with viral DNA to transfer

the expression cassette to the polyhedrin locus of the viral DNA. The polyhedrin coding sequences

have been replaced by a multiple cloning site with 18 unique sites that facilitate the insertion of

foreign genes in the correct orientation for expression. The Pac I site at the end of the MCS region

provides translational stop codons in all three reading frames for expression of truncated proteins.

pBacPAK8 has a pUC origin of replication, an M13 origin for single-stranded DNA production, and

an ampicillin resistance gene for selection in E. coli.

pBacPAK8昆蟲桿狀病毒高效表達(dá)載體，帶有AcMNPV polyhedrin強(qiáng)啟動子。表達(dá)盒整合在桿狀病毒DNA的polyhedrin位點，PacI位點含有終止密碼子適于三種讀碼框，pUC復(fù)制子，M13起始位點用于單鏈DNA生成，Amp抗性。BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心提供.png

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