pRB473 plasmid

E. coli-Staphylococcusshuttle vector pRB473, a derivative of plasmid pRB373  in which thepUB110 kanamycin resistance gene is replaced by the chloramphenicol acetyltransferase(cat) gene.

pRB373 plasmid

Hosts : (E.coli)(E.coli HB101)(Bacillus subtilis)(B.subtilis DB403)

Misc.Comments : Deposited by: Reinhold Bruckner Shuttle vector. [1] Neo confers resistance to neomycin and kanamycin and ble confers resistance to bleomycin and phleomycin. [1] Structural stability of the plasmid in B. subtilis can be affected by high levels of protein production. Under these conditions, cell growth and stability may be improved by reducing the antibiotic concentration in the media. [1] May not be suitable for cloning very strong expression signals. [1] The order of the major features in the plasmid is: To terminator - HindIII/MCS/EcoRI - rrnB terminator - ampR - pMB1 ori - pUB110 ori - neoR- bleR. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--5.8; BglI/BglII--3.5, 2.3; BamHI--5.8. (ATCC staff) Medium is 1227 LB plus ampicillin.

Sequence information:

ID   PRB373     preliminary; circular DNA; SYN; 5800 BP.
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AC   ATCC77373;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/E.coli plasmid vector pRB373 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRB373 from pRB273 & pUC18
RC   pRB374 from pRB373 & vegII
RC   pRB394 from pRB373 & pCR94
RC   pRB395 from pRB394 & vegII
RC   pRB381 from pRB373 & pMC1871
RC   pRB382 from pRB381 & vegII
RC   pHS1, pHS2, pEC1, pBL5, pBL6 from pRB series
RA   Bruckner R.;
RT   "A series of shuttle vectors for Bacillus subtilis and Escherichia
RT   coli";
RL   Gene 122:187-192(1992).
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RN   [2]
RC   pCR94, pCR1886, pCR198, pCR1809, pCR1807 from pUB112 & pRB273
RC   pCR2, pCR5, pCR54, pCR125 from pUB112 & pRB273
RC   pCS1837, pCS1827, pCS1825, pCS1808, pCS1816 from pUB112 & pRB273
RA   Bruckner R., Matzura H.;
RT   "Regulation of the inducible chloramphenicol transferase gene of
RT   the Staphylococcus aureus plasmid pUB112";
RL   EMBO J. 4:2295-2300(1985).
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CC   Deposited by: Reinhold Bruckner
CC   Shuttle vector. [1]
CC   Neo confers resistance to neomycin and kanamycin and ble confers
CC   resistance to bleomycin and phleomycin. [1]
CC   Structural stability of the plasmid in B. subtilis can be affected by
CC   high levels of protein production.  Under these conditions, cell
CC   growth and stability may be improved by reducing the antibiotic
CC   concentration in the media. [1]
CC   May not be suitable for cloning very strong expression signals. [1]
CC   The order of the major features in the plasmid is: To terminator -
CC   HindIII/MCS/EcoRI - rrnB terminator - ampR - pMB1 ori - pUB110 ori -
CC   neoR- bleR. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--5.8; BglI/BglII--3.5, 2.3; BamHI--5.8. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pRB373)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(E.coli HB101)(Bacillus subtilis)(B.subtilis DB403)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pRB273 5600bp, in MCS
FT                   2. E. coli 200bp, rrnB gene T1
FT                   -> plasmid 5800bp
FT                   1. plasmid 5800bp
FT                   2. pUC18, MCS
FT                   -> pRB373 5800bp"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-
FT                   SalI-AccI-PstI-SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT Bacillus neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
FT   CDS             0..0
FT                   /note="ANT Bacillus bleomycin resistance gene (ble)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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