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pDule-tfmF plasmid
pDule-tfmF蛋白定點氟化標記質粒載體,利用氨酰tRNA合成酶/tRNA CUA將L-4-triuoromethylphenylalanine(tfmF)標記到蛋白特定氨基酸,可用于蛋白構象檢測等.
Map:
F and pDule plasmid.(a) The chemical structure of tfmF. (b) pDule-tfmF plasmid. The tfmF-specific aminoacyl-tRNA synthetase is expressed between an lpp promoter and an rrnB terminator. The tRNACUA is expressed between an lpp promoter and an rrnC terminator. The plasmid contains a p15A origin and a tetracycline resistance marker.
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