NZ3900乳酸菌菌株

Product Description and Application

? Lactococcus lactis strain NZ3900, food grade. Host strain for NICE? Lactococcus gene expression

systems.

For handling carefully read the handbook pertaining to the gene expression system.

Care must be taken not to interrupt the cooling chain. Always store frozen stock at -80 °C or lower.

Genotype

? lacF-, pepN::nisRnisK

Contents

? 1 ml cell suspension in growth medium supplemented with 10 - 30% glycerol.

Host strains

All supplied strains are derivatives of L.lactis subsp. cremorisMG1363, a plasmid-free progeny of the dairy starterstrain NCDO712.

The most commonly used host strain isNZ9000. To construct this strain the genes for NisK and NisR were integratedinto the pepN gene of MG1363.The two genes are

transcribed from their own constitutivepromoter.

Lactococcus lactis NZ9000 – pepN::nisRnisK;

Standard host strain for nisin regulated gene expression(NICE). The strain contains the regulatory genes nisR and nisK integrated into the pepN gene (broad range aminopeptidase) (Kuipers et al., 1998; Mierau andKleerebezem, 2005).

BioVector NTCC質粒載體菌種細胞基因保藏中心