| 出品公司: | Promega |
|---|---|
| 載體名稱: | pGL4.70 ; pGL4.70[hRluc] |
| 質粒類型: | 信號通路報告載體；哺乳動物載體；海腎熒光素酶報告載體 |
| 高拷貝/低拷貝: | -- |
| 克隆方法: | 限制性內切酶，多克隆位點 |
| 啟動子: | 無 |
| 載體大小: | 3522 bp |
| 5' 測序引物及序列: | -- |
| 3' 測序引物及序列: | -- |
| 載體標簽: | hRluc |
| 載體抗性: | 氨芐青霉素 |
| 篩選標記: | 潮霉素(Hygromycin) |
| 克隆菌株: | TOP10等常規菌株 |
| 宿主細胞（系）: | HEK293等 |
| 備注: | pGL4.70[hRluc]載體是信號通路報告載體；無啟動子；含海腎 熒光素酶報告基因hRluc。 |
| 產品目錄號: | E6881 |
| 穩定性: | 瞬表達 |
| 組成型/誘導型: | 組成型 |
| 病毒/非病毒: | 非病毒 |
pGL4.70[hRluc]信號通路報告載體；哺乳動物載體；海腎熒光素酶報告載體-BioVector NTCC Inc.
- 價 格：￥8920
- 貨 號：pGL4.70[hRluc]
- 產 地：北京
- BioVector NTCC典型培養物保藏中心
- 聯系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作QQ:1843439339 (微信同號)
郵件：Biovector@163.com
手機：18901268599
地址：北京
- 已注冊
pGL4.70[hRluc]載體-BioVector NTCC質粒載體菌種細胞基因保藏中心
pGL4.70載體基本信息
pGL4.70載體質粒圖譜和多克隆位點信息
pGL4.70載體簡介
The pGL4.70[hRluc] Vector encodes the hRluc gene (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.70[hRluc] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows for cloning of a promoter of choice.
pGL4.70載體序列
LOCUS AY738226 3522 bp DNA circular SYN 08-NOV-2004
DEFINITION Renilla luciferase reporter vector pGL4.70[hRluc], complete
sequence.
ACCESSION AY738226
VERSION AY738226.1 GI:55535630
KEYWORDS .
SOURCE Renilla luciferase reporter vector pGL4.70[hRluc]
ORGANISM Renilla luciferase reporter vector pGL4.70[hRluc]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3522)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
Corporation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3522)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-2004) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..3522
/organism="Renilla luciferase reporter vector
pGL4.70[hRluc]"
/mol_type="other DNA"
/db_xref="taxon:296663"
misc_feature 1..70
/note="multiple cloning site region"
CDS 100..1035
/codon_start=1
/transl_table=11
/product="luciferase hRluc2"
/protein_id="AAV52877.1"
/db_xref="GI:55535631"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
misc_feature 1067..1288
/note="SV40 late poly(A) region"
primer_bind 1356..1375
/note="reporter vector primer 4 (RVPrimer4)"
rep_origin 1613
/note="ColE1-derived"
gene complement(2404..3264)
/gene="ampR"
CDS complement(2404..3264)
/gene="ampR"
/note="AmpR"
/codon_start=1
/transl_table=11
/product="synthetic beta-lactamase"
/protein_id="AAV52878.1"
/db_xref="GI:55535632"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3369..3522
/regulatory_class="polyA_signal_sequence"
/note="synthetic; transcriptional pause site"
primer_bind complement(3471..3490)
/note="reporter vector primer 3 (RVPrimer3)"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
1021 aagaacgagc agtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
1081 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
1141 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
1201 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
1261 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt
1321 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
1381 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
1441 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
1501 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
1561 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
1621 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
1681 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
1741 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
1801 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
1861 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
1921 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
1981 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
2041 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
2101 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
2161 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
2221 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
2281 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
2341 tctaaagtat atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag
2401 tggttaccag tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc
2461 catagtggcc tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg
2521 ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat
2581 gaaccagcca gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat
2641 ccagtctatg agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg
2701 aagagttgtg gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc
2761 gttcaactct ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa
2821 tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc
2881 gctcatggta atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt
2941 ttccgtgacc ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag
3001 ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt
3061 gctcatcatc gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag
3121 atccagttcg atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac
3181 cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc
3241 gacacgaaaa tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca
3301 gggttactag tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga
3361 caggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
3421 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
3481 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ct
//- 公告/新聞
