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首頁 ? Human Dental Follicle stem Cells hDFSC Cells人牙濾泡干細胞-BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

Human Dental Follicle stem Cells hDFSC Cells人牙濾泡干細胞-BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

價　　格：￥99850
貨　　號：NTCC?-HDFSC
產　　地：北京

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Human Dental Follicle stem Cells hDFSC Cells人牙濾泡干細胞

BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

Culture Medium	Alpha MEM, w: 2.0 mM stable Glutamine, w/o: Ribonucleosides, w/o: Deoxyribonucleosides, w: 1.0 mM Sodium pyruvate, w: 2.2g/L NaHCO3
Medium supplements	Supplement the medium with 10% FBS, 2 ng/ml bFGF
Passaging solution	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	2 x 10^4 cells/cm^2
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	Human Dental Follicle stem cells (DFSC, hDFSC) are shipped in a deep-frozen state on dry ice. Upon receipt, confirm that the vial remains frozen. For storage, place the cryovial immediately at temperatures below -150 degrees. If you plan to culture the cells immediately, swiftly thaw the vial by shaking it in a 37 degrees water bath with clean water and an antimicrobial agent for 40-60 seconds. Remove the vial once a small ice clump persists, ensuring it remains cold. Proceed with all subsequent steps under aseptic conditions. In a sterile flow hood, disinfect the cryovial with 70% ethanol. Then, gently open the vial and transfer the cell suspension into a 15 ml centrifuge tube pre-filled with 8 ml of room temperature culture medium. Gently mix the cells. For cell separation, centrifuge at 300 x g for 3 minutes and dispose of the supernatant. Skipping centrifugation is optional, although any residual freezing medium should be removed after 24 hours. Resuspend the pellet gently in 10 ml of fresh culture medium and divide between two T25 culture flasks. Follow the subculture protocol for subsequent steps.

Sterility	Mycoplasma contamination is rigorously excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Human Dental Follicle stem Cells (hDFSC) Cells

Human Dental Follicle stem Cells (hDFSC) Cells

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IPsale?生物知識產權出售平臺: 2024-06-27
BioVector NTCC Inc.產品分類中篇1: 2014-12-22
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