pCM130/tac plasmid vector廣宿主表達質粒載體

BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

Map：

Vector backbone pCM62

Backbone manufacturerLidstrom Lab (BioVector?-045826)

Backbone size (bp)7965

Modifications to backbonecreated by inserting a 1.0 kb fragment containing xylE (from pCM75) into pCM62 cut with AflIII and HindIII, followed by the insertion of a 0.5 kb XbaI-XmnI trrnB fragment from pMTL20T1T2 into the blunted EcoRI site.

Vector typelow-background broad-host-range promoter-probe vector using xylE as a reporter gene

Promoter：tac promoter

GROWTH IN BACTERIA

Bacterial Resistance(s)Tetracycline, 10 μg/mL

Growth Temperature37°C

Growth Strain(s)DH5alpha

Copy numberUnknown

XylE replaces the lac promoter in pCM62 (BioVector?-045826) and is used as a reporter to test promoter activity. A transcriptional terminator was added to reduce background activity.