[Designation名稱] NTCC? ID HCT 116 CMV-OsTIR1 Cell Line細(xì)胞株
BioVector NTCC Inc.

DR cancercelllines; CVCL_RJ11
DR RCB; RCB4662
SY HCT116 CMV-OsTIR1
DR Wikidata; Q54881927
RX PubMed=27052166;
CC Population: Caucasian.
CC Characteristics: Parent cell line to study the rapid depletion of a protein. The expressed rice TIR1 can form a functional SCF(OsTIR1) E3 ligase complex with the endogenous components in human cells. In the presence of auxin, a protein of interest fused with mAID is rapidly degraded upon poly-ubiquitylation.
CC Characteristics: The CMV-OsTIR1-PRO (pMK232) vector was introduced in both alleles of the AAVS1 locus by using CRISPR/Cas9.
CC Sequence variation: Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
CC Sequence variation: Mutation; HGNC; 1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
CC Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
CC Sequence variation: Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
CC Sequence variation: Mutation; HGNC; 3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
[Organism物種來(lái)源]
[Disease疾病種類]
[Accession編號(hào)] NTCC?-AC CVCL_RJ11
[Comments描述]
[Reference參考文獻(xiàn)]
[Category分類]
[Date保藏時(shí)間]
[Synonyms別名]
[Hierarchy]
培養(yǎng)方法：
Medium培養(yǎng)基：NTCC? ID HCT 116 CMV-OsTIR1 Complete Medium
Growth properties形態(tài)：貼壁/懸?。?br/>Culture Condition培養(yǎng)條件：Temperature溫度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生產(chǎn)廠家: BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心
Website網(wǎng)址：www.biovector.net