[Designation名稱] NTCC? ID HEC-LTT Cell Line細胞株
BioVector NTCC Inc.

DR Wikidata; Q107115253
RX PubMed=19705962;
SY HUVEC Uni-TAg/bi-hTert
RX PubMed=26345505;
CC Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
CC Characteristics: In the presence of doxycycline, hTERT and SV40-TAg expression are activated, resulting in high cell proliferation and unlimited expansion. In the absence of the doxycycline, cells stop proliferating and can be maintained in a growth-arrested state that reflects the status of endothelial cells in vivo (PubMed=26345505).
CC Transfected with: HGNC; 11730; TERT.
CC Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
CC Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC Derived from site: In situ; Umbilical vein; UBERON=UBERON_0002066.
[Organism物種來源]
[Disease疾病種類]
[Accession編號] NTCC?-AC CVCL_A7FW
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[Reference參考文獻]
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[Date保藏時間]
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培養(yǎng)方法：
Medium培養(yǎng)基：NTCC? ID HEC-LTT Complete Medium
Growth properties形態(tài)：貼壁/懸浮：
Culture Condition培養(yǎng)條件：Temperature溫度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生產廠家: BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心
Website網址：www.biovector.net