[Designation名稱] NTCC? ID HEK293-DR-GFP-XRCC3-5/X3+ Cell Line細胞株
BioVector NTCC Inc.

DR DSMZCellDive; ACC-854
DR Wikidata; Q94095087
DR DSMZ; ACC-854
CC Characteristics: The parental XRCC3 KO cell line is functionally reconstituted by retroviral transfection of XRCC3.
CC Characteristics: Transfected with the hprtDR-GFP reporter construct that targets the DR-GFP into the hprt locus and includes a dominant selectable puroR marker. The DR-GFP construct which contains a tandem repeat of two inactive genes coding for EGFP. After transfection with an expression vector coding for the I-SceI enzyme, recombination induced by the cleavage of the I-SceI site recreates a functional EGFP.
CC Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
CC Doubling time: ~20-30 hours (DSMZ=ACC-854).
CC Knockout cell: Method=CRISPR/Cas9; HGNC; 12830; XRCC3.
CC Transfected with: HGNC; 12830; XRCC3.
CC Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
CC Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
[Organism物種來源]
[Disease疾病種類]
[Accession編號] NTCC?-AC CVCL_XX10
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培養方法：
Medium培養基：NTCC? ID HEK293-DR-GFP-XRCC3-5/X3+ Complete Medium
Growth properties形態：貼壁/懸浮：
Culture Condition培養條件：Temperature溫度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生產廠家: BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心
Website網址：www.biovector.net