Cell Line

hPSCreg name NTCC? UOSe015-A

Cite as: UOSe015-A(NTCC?_BW83)

Alternative name(s)

MasterShef11, MstrShef11, MShef11

Cell line typeHuman embryonic stem cell (hESC)

Similar lines No similar lines found.

Last update 22ndJanuary 2022

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Provider

GeneratorUniversityof Sheffield (UOS)

Contact:

Centre for Stem Cell Biology

OwnerCentrefor Stem Cell Biology

Distributors

University of Sheffield (UOS)

Derivation countryUnited Kingdom

External Databases

BioSamplesSAMEA104627969

CellosaurusCVCL_BW83

WikidataQ54904044

General Information

Publications

Canham MA et al. The Molecular Karyotype of25 Clinical-Grade Human Embryonic Stem Cell Lines. Scientific reports. 2015 Nov26;5:17258.

Merkle FT et al. Human pluripotent stemcells recurrently acquire and expand dominant negative P53 mutations. Nature.2017 May 11;545(7653):229-233.

Halliwell JA et al. Nucleosides RescueReplication-Mediated Genome Instability of Human Pluripotent Stem Cells. Stemcell reports. 2020 Jun 9;14(6):1009-1017.

Projects

TempoReg: Molecular and metabolic pathwayscontrolling developmental timing.

* Is the cell line readily obtainable forthird parties?

Yes

Research use: allowed

Clinical use: allowed

Commercial use: allowed

Donor Information

General Donor Information

Sexmale

Phenotype And Disease Related Information(Donor)

DiseasesNodisease was diagnosed.

Family historyNo

Is the medical history available uponrequest?No

Is clinical information available?No

Karyotyping (Donor)

Has the donor karyotype been analysed?

No

Other Genotyping (Donor)

Is there genome-wide genotyping orfunctional data available?

No

External Databases (Donor)

BioSamplesSAMEA104627970

Ethics

Was the embryo established purely forresearch purposes?No

Have both parents consented to the use ofthe embryo for ESC derivation?Yes

Has informed consent been obtained from thedonor of the embryo/tissue from which the pluripotent stem cells have beenderived?Yes

Was the consent voluntarily given?Yes

Has the donor been informed thatparticipation will not directly influence their personal treatment?Yes

Can you provide us with a copy of the DonorInformation Sheet provided to the donor?Yes

Do you (Depositor/Provider) hold theoriginal Donor Consent Form?No

If you do not hold the Donor Consent Form,do you know who does?Yes

Contact information / weblinkhttps://jessopfertility.org.uk/

Alternatives to consent are available?Yes

Alternatives to consent

Alternative consent approval number

Please indicate whether the data associatedwith the donated material has been pseudonymised or anonymised.pseudonymised

Does consent explicitly allow thederivation of pluripotent stem cells?Yes

Does consent expressly prevent thederivation of pluripotent stem cells?No

Does consent prevent CELLS DERIVED FROM THEDONATED BIOSAMPLE from being made available to researchers anywhere in theworld?No

How may genetic information associated withthe cell line be accessed?No information

Will the donor expect to receive financialbenefit, beyond reasonable expenses, in return for donating the biosample?No

Has a favourable opinion been obtained froma research ethics committee, or other ethics review panel, in relation to theResearch Protocol including the consent provisions?Yes

Name of accrediting authority involved?National Research Ethics Service - North WestLondon Rec-2

Approval number05/Q0501/63

HESC Derivation

Date of derivation2012-06-21

Embryo stageBlastula with ICM and Trophoblast

Supernumerary embryos from IVF treatment?

Yes

Separation of research and IVF treatment?

Yes

PGD Embryo?

No

Expansion status4

4

Stephenson 2007

ICM morphologyB

B

Stephenson 2007

Trophectoderm morphologya

a

Stephenson 2007

ZP removal techniqueSpontaneous Hatching

Cell isolationMechanical

Cell seedingEmbryo

Derived under xeno-free conditions?

Yes

Derivation under GMP?

Yes

Available as clinical grade?

Yes

Culture Conditions

Surface coatingLaminin-511 then CellSTART

Feeder cells

No

Passage methodMechanically

O2 Concentration20 %

CO2 Concentration5 %

MediumOthermedium:

Base medium: Nutristem

Main protein source: Human Serum Albumin

Serum concentration: 5 %

NutriStem-protocol.pdf

Has Rock inhibitor (Y27632) been used atpassage previously with this cell line?

No

Has Rock inhibitor (Y27632) been used atcryo previously with this cell line?

No

Has Rock inhibitor (Y27632) been used atthaw previously with this cell line?

No

Characterisation

Analysis Of Undifferentiated Cells

Marker Expression

MarkerExpressedImmunostainingRT-PCRFlow CytometryEnzymatic AssayExpression Profiles

SSEA-1

No

SSEA-3

Yes

SSEA-4

Yes

TRA 1-60

Yes

TRA 1-81

Yes

NANOG

Yes

POU5F1 (OCT-4)

Yes

Morphology

Morphology pictures

Differentiation Potency

Endoderm

Endoderm

Ont Id: UBERON_0000925

In vitro spontaneous differentiation

MarkerExpressed

alpha fetal protein

Yes

Mesoderm

Mesoderm

Ont Id: UBERON_0000926

In vitro spontaneous differentiation

MarkerExpressed

Desmin

Yes

Ectoderm

Ectoderm

Ont Id: UBERON_0000924

In vitro spontaneous differentiation

MarkerExpressed

Beta Tubulin III

Yes

Microbiology / Virus Screening

MycoplasmaNegative

Certificate Of Analysis

Is there a certificate of analysisavailable?

Yes

Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?

Yes

46, XY

MstrShef11_-_Karyotype_Bank_Trace_Diagram_V2.pdf

Passage number: P3

Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping orfunctional data available?

Yes

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