hPSCreg nameUMANe004-A

Cite as: UMANe004-A(RRID:CVCL_BW69)

Alternative name(s)

Man-15

Cell line typeHuman embryonic stem cell (hESC)

Similar linesNo similar lines found.

Last update22ndMay 2019

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Provider

GeneratorUniversityof Manchester (UMAN)

Contact:

Professor Sue Kimber - North West EmbryonicStem Cell Centre

OwnerUniversityof Manchester (UMAN)

Distributors

National Institute for Biological Standardsand Control - UK Stem Cell Bank (USCB)

Derivation countryUnited Kingdom

External Databases

BioSamplesSAMEA104576524

CellosaurusCVCL_BW69

WikidataQ54903991

General Information

Publications

Ye J et al. High quality clinical gradehuman embryonic stem cell lines derived from fresh discarded embryos. Stem cellresearch & therapy. 2017 Jun 5;8(1):128. (reference publication)

* Is the cell line readily obtainable forthird parties?

Yes

Clinical use: allowed

Commercial use: not allowed

Donor Information

General Donor Information

Sexmale

Phenotype And Disease Related Information(Donor)

DiseasesNodisease was diagnosed.

Is the medical history available uponrequest?Yes

Is clinical information available?Yes

Karyotyping (Donor)

Has the donor karyotype been analysed?

Yes

Normal Male 46 XY

Karyogram_Man-15.pdf

Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping orfunctional data available?

Yes

Array CGH

External Databases (Donor)

BioSamplesSAMEA104575906

Ethics

Was the embryo established purely forresearch purposes?No

Have both parents consented to the use ofthe embryo for ESC derivation?Yes

Has informed consent been obtained from thedonor of the embryo/tissue from which the pluripotent stem cells have beenderived?Yes

Was the consent voluntarily given?Yes

Alternatives to consent are available?Yes

Alternatives to consent

Please indicate whether the data associatedwith the donated material has been pseudonymised or anonymised.pseudonymised

Does consent explicitly allow thederivation of pluripotent stem cells?Yes

Does consent expressly prevent thederivation of pluripotent stem cells?No

Will the donor expect to receive financialbenefit, beyond reasonable expenses, in return for donating the biosample?No

Has a favourable opinion been obtained froma research ethics committee, or other ethics review panel, in relation to theResearch Protocol including the consent provisions?Yes

Name of accrediting authority involved?HTA, HFEA, and UK Stem Cell Bank

Approval numberHTA Licence number: 22627, HFEA Licence number: R0171-4-a. UKSCBapproval number: SCSC14-24

HESC Derivation

Date of derivation2011-10-03

Embryo stageBlastula with ICM and Trophoblast

Supernumerary embryos from IVF treatment?

Yes

Separation of research and IVF treatment?

Yes

PGD Embryo?

No

Expansion status2

2

Stephenson 2007

ICM morphologyC

C

Stephenson 2007

Trophectoderm morphologyg

g

Stephenson 2007

ZP removal techniqueChemical

Cell isolationMechanical

Cell seedingIsolated ICM

Derived under xeno-free conditions?

Yes

Derivation under GMP?

Yes

Available as clinical grade?

Yes

Culture Conditions

Surface coatingCELLstart

Feeder cellsHuman Dermal Fibroblast

Cellfinder Ont Id: CELDA_000001419

Passage methodMechanically

O2 Concentration20 %

CO2 Concentration5 %

MediummTeSR?2

Supplements

SupplementAmountUnit

Penicillin/streptomycin1%

L-Glutamine2mM

Has Rock inhibitor (Y27632) been used atpassage previously with this cell line?

Unknown

Has Rock inhibitor (Y27632) been used atcryo previously with this cell line?

Unknown

Has Rock inhibitor (Y27632) been used atthaw previously with this cell line?

Unknown

Characterisation

Analysis Of Undifferentiated Cells

Marker Expression

MarkerExpressedImmunostainingRT-PCRFlow CytometryEnzymatic AssayExpression Profiles

NANOG

Yes

POU5F1 (OCT-4)

Yes

SOX2

Yes

SSEA-1

No

SSEA-4

Yes

TRA 1-60

Yes

EpiPluriScore

Score:

MarkerPresentAbsent

mCpG

OCT4

Differentiation Potency

Endoderm

ESC Derived Cell Line

Ont Id: EFO_0005738

In vitro spontaneous differentiation

MarkerExpressed

GATA Binding Protein 6

Yes

FOXA2

Yes

Intestine

Ont Id: UBERON_0000160

In vivo teratoma

MarkerExpressed

Hematoxylin and eosin stain

Yes

Mesoderm

ESC Derived Cell Line

Ont Id: EFO_0005738

In vitro spontaneous differentiation

MarkerExpressed

Actin, Alpha 2, Smooth Muscle, Aorta

Yes

Vimentin

Yes

Skeletal Muscle Tissue

Ont Id: UBERON_0001134

In vivo teratoma

MarkerExpressed

Hematoxylin and eosin stain

Yes

Ectoderm

ESC Derived Cell Line

Ont Id: EFO_0005738

In vitro spontaneous differentiation

MarkerExpressed

Tubulin Beta 3 Class III

Yes

Neurofilament, light polypeptide

Yes

Neural Retinal Epithelium

Ont Id: EHDAA2_0001253

In vivo teratoma

MarkerExpressed

Hematoxylin and eosin stain

Yes

Microbiology / Virus Screening

HIV 1Negative

HIV 2Negative

Hepatitis BNegative

Hepatitis CNegative

MycoplasmaNegative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?

Yes

Normal Male 46 XY

Tissue_Cell_Culture_Report_Man-15.pdf

Passage number: 16

Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping orfunctional data available?

Yes

Array CGH

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