Name細(xì)胞名稱：NTCC? GM15876 XERODERMA PIGMENTOSUM, COMPLEMENTATION GROUP A; XPA Skin, Unspecified Cell Line
色素性干皮病，補(bǔ)充組 A； XPA細(xì)胞株
Description描述：The cell line (also known as XP20S-pCAH19WS) is a derivative of the SV40 Transformed and immortalized xeroderma pigmentosum complementation group A fibroblast XP20S (GM04312), which was subsequently transfected with the full length cDNA of the XPAC gene to correct the abnormal phenotype; GM15876A shows normal UV sensitivity, judged by survival of UV damaged Shuttle vector plasmid, restoration of transcription of a UV damaged CAT expression vector, and by a return to the normal spectrum of shuttle vector mutations found in control cells (Levy et al, Carcinogenesis 16:1557-63,1995); the full length XPAC cDNA is stably expressed using the cytomegalovirus enhancer and chicken B-actin promoter (Miyamoto et al, J Biol Chem 267:12182-87,1992), and the XPA protein in GM15876A is over expressed by a factor of 14 compared with the normal, repair-competent SV40 Transformed GM00637 (Levy et al, Carcinogenesis 16:15757-63,1995); the corrected phenotype of GM15876A is stable; selection for the XPAC gene is not required
Category分類：Fibroblast
ID編號(hào):NTCC?-GM15876
Size/Quantity數(shù)量: 1 Flask/2 Vials
Biosafety Level生物安全級(jí)別:1
Shipping Info運(yùn)輸方式: RT/Dry Ice
Storage儲(chǔ)存方式: RT/Liquid Nitrogen
Race: Asian
Age年齡:7 YR
Gender性別:Female
Cell Type細(xì)胞類型: XERODERMA PIGMENTOSUM, COMPLEMENTATION GROUP A; XPA
Affected State:Yes
Product:Fibroblast
Gene:
Mutations突變:
Ethnicity:
Family:695
Relationship:proband
Karyotype:
Tissue Type組織來(lái)源:Skin
Complete Growth Medium完全培養(yǎng)基: BioVector? Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent Complete Medium
Subculturing傳代方法:
Cryopreservation凍存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培養(yǎng)條件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鑒定數(shù)據(jù):
References參考文獻(xiàn):

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