Name細(xì)胞名稱(chēng)：NTCC? GM04250 RETINOBLASTOMA, SPORADIC Skin, Arm Cell Line
散發(fā)性視網(wǎng)膜母細(xì)胞瘤細(xì)胞株
Description描述：Clinically affected; diagnosed at 3 months of age with bilateral retinoblastoma; poor weight gain, weak sucking, cyanosis, and abnormal eye movement; slight normochromic anemia; developmental retardation; gross ophthalmological exam revealed leukocoria and sluggish pupillary light response in right eye; detailed exam of the right fundus showed a solid tumor with total retinal detachment, and the left fundus showed two elevated tumor masses; histological examination of the enucleated right eye revealed a well-differentiated retinoblastoma with focal necrosis and numerous rosette formation; procedures include: enucleation of right eye, transscleral cryocoagulation of the left eye, photocoagulation, and radiation; translocation of the long arm of chromosome 13 to the short arm of the X chromosome, karyotype: 46,XX,t(X;13)(p22; q13) in leukocytes; translocated X found to be preferentially inactivated in skin fibroblasts with the inactivation spreading over the translocated chromosome 13; chromosome studies of the parents and sibling revealed no abnormalities; erythrocyte esterase D type 1-1 identified in the proband, sibling and both parents.
Category分類(lèi)：Fibroblast
ID編號(hào):NTCC?-GM04250
Size/Quantity數(shù)量: 1 Flask/2 Vials
Biosafety Level生物安全級(jí)別:1
Shipping Info運(yùn)輸方式: RT/Dry Ice
Storage儲(chǔ)存方式: RT/Liquid Nitrogen
Race: Asian
Age年齡:2 YR
Gender性別:Female
Cell Type細(xì)胞類(lèi)型: RETINOBLASTOMA, SPORADIC
Affected State:Yes
Product:Fibroblast
Gene:
Mutations突變:
Ethnicity:JAPANESE
Family:
Relationship:proband
Karyotype:arr 2q13(110212011-110519397)x3
Tissue Type組織來(lái)源:Skin
Complete Growth Medium完全培養(yǎng)基: BioVector? Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent Complete Medium
Subculturing傳代方法:
Cryopreservation凍存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培養(yǎng)條件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鑒定數(shù)據(jù):
References參考文獻(xiàn):

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