Name細胞名稱：NTCC? GM04339 CYSTIC FIBROSIS; CF | CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; CFTR Cell Line
囊性纖維化; CF |囊性纖維化跨膜電導調(diào)節(jié)器； CFTR細胞株
Description描述：Advanced chronic pulmonary disease; no pancreatic involvement; elevated sweat electrolytes; a sib died at age 4 with meconium ileus; defective fibroblast cAMP-stimulated Cl- channel; donor subject is a compound heterozygote: one allele carries the deltaF508 deletion mutation (PHE508DEL) and a second allele carries a G-to-A substitution at nucleotide 386 (386G>A) which converts the gly-85 codon (GGA) to a glu (GAA), resulting in a missense mutation in exon 3 in the CFTR gene [GLY85GLU (G85E)]
Category分類：Fibroblast
ID編號:NTCC?-GM04339
Size/Quantity數(shù)量: 1 Flask/2 Vials
Biosafety Level生物安全級別:1
Shipping Info運輸方式: RT/Dry Ice
Storage儲存方式: RT/Liquid Nitrogen
Race: White
Age年齡:24 YR
Gender性別:Male
Cell Type細胞類型: CYSTIC FIBROSIS; CF | CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; CFTR
Affected State:Yes
Product:Fibroblast
Gene:CFTR
Mutations突變:GLY85GLU PHE508DEL
Ethnicity:
Family:706
Relationship:proband
Karyotype:
Tissue Type組織來源:
Complete Growth Medium完全培養(yǎng)基: BioVector? Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent Complete Medium
Subculturing傳代方法:
Cryopreservation凍存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培養(yǎng)條件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鑒定數(shù)據(jù):
References參考文獻:

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