Name細(xì)胞名稱(chēng)：NTCC? GM04345 CYSTIC FIBROSIS; CF | CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; CFTR B-Lymphocyte Cell Line
囊性纖維化; CF |囊性纖維化跨膜電導(dǎo)調(diào)節(jié)器； CFTR細(xì)胞株
Description描述：Classic CF with pancreatic insufficiency & diabetes mellitus; elevated sweat electrolytes; see GM04346 Fibroblast; donor subject is a compound heterozygote: one allele carries the deltaF508 deletion mutation (PHE508DEL) and a second allele carries a G-to-A substitution at nucleotide 3978 (3978G>A) which converts the trp-1282 codon (TGG) to a stop codon (TGA), resulting in a nonsense mutation in exon 20 in the CFTR gene [TRP1282TER (W1282X)]
Category分類(lèi)：B-Lymphocyte
ID編號(hào):NTCC?-GM04345
Size/Quantity數(shù)量: 1 Flask/2 Vials
Biosafety Level生物安全級(jí)別:1
Shipping Info運(yùn)輸方式: RT/Dry Ice
Storage儲(chǔ)存方式: RT/Liquid Nitrogen
Race: White
Age年齡:25 YR
Gender性別:Male
Cell Type細(xì)胞類(lèi)型: CYSTIC FIBROSIS; CF | CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; CFTR
Affected State:Yes
Product:LCL
Gene:CFTR
Mutations突變:PHE508DEL TRP1282TER
Ethnicity:
Family:712
Relationship:proband
Karyotype:
Tissue Type組織來(lái)源:Blood
Complete Growth Medium完全培養(yǎng)基: BioVector? Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent Complete Medium
Subculturing傳代方法:
Cryopreservation凍存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培養(yǎng)條件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鑒定數(shù)據(jù):
References參考文獻(xiàn):

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