Name細胞名稱：NTCC? AG08031 EQUUS | ORDER: PERISSODACTYLA Aorta, thoracic Cell Line
雅科仕 |目： 奇蹄目細胞株
Description描述：Standardbred horse. The thoracic aorta was removed 1 hour post-mortem on 2/27/85. Strips of the medial layer were minced, treated with collagenase and then elastase. Cells released by this treatment were used for the primary culture. Cell morphology is fibroblast-like and the cells form layered sheets at post-confluence. The karyotype is 64,XX; normal diploid female. The culture tested positive for alpha-smooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluorescence assays). Other cultures from this horse are AG08029 and AG08030. The legacy karyotype description shown in this Remark may not be representative of the current available product.
Category分類：Smooth muscle
ID編號:NTCC?-AG08031
Size/Quantity數量: 1 Flask/2 Vials
Biosafety Level生物安全級別:1
Shipping Info運輸方式: RT/Dry Ice
Storage儲存方式: RT/Liquid Nitrogen
Race:
Age年齡:2 YR
Gender性別:Female
Cell Type細胞類型: EQUUS | ORDER: PERISSODACTYLA
Affected State:No
Product:Smooth muscle
Gene:
Mutations突變:
Ethnicity:
Family:1093
Relationship:proband
Karyotype:64,XX
Tissue Type組織來源:Artery
Complete Growth Medium完全培養基: BioVector? MEM (Eagle) with 2X conc. A.A. and VIT./MCDB110, 1:1 mixture with 2mM L-glutamine or equivalent Complete Medium
Subculturing傳代方法:
Cryopreservation凍存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培養條件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鑒定數據:
References參考文獻:

Supplier供應商:BioVector質粒載體菌株細胞蛋白抗體基因保藏中心
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